mouse il-4 biotinylated antibody  (Bio-Techne corporation)

Journal: PLoS ONE

Article Title: Alternaria-Induced Release of IL-18 from Damaged Airway Epithelial Cells: An NF-κB Dependent Mechanism of Th2 Differentiation?

doi: 10.1371/journal.pone.0030280

Figure Lengend Snippet: ( A ) Effect of IL-18 on IL-4 secreting splenocytes ELISPOTS. ( B ) T cell differentiation protocol used in Panels 3C–E and . ( C, D and E ) Effect of IL-18 or IL-4 on Th2 differentiation of naïve CD4+ T-cells isolated from WT mice ( C ), IL-4 −/− mice ( D ) and WT and STAT6 −/− mice ( E ). n = 5−7 per group.

Article Snippet: Plates were then washed and overlaid with biotinylated anti-IL-4 (clone BVD6- 24G2, Endogen), washed, and treated with a 1∶2000 dilution of avidin conjugated alkaline phosphatase (Vector Laboratories, Burlingame, CA) for 2 hrs at room temperature.

Techniques: Cell Differentiation, Isolation

Journal: PLoS ONE

Article Title: Alternaria-Induced Release of IL-18 from Damaged Airway Epithelial Cells: An NF-κB Dependent Mechanism of Th2 Differentiation?

doi: 10.1371/journal.pone.0030280

Figure Lengend Snippet: CD4 + T-cells from Wild-type (WT) and NF-κB p50 −/− mice were cultured with IL-18 in the differentiation phase, restimulated with con A, and secretion of IL-4 ( A ), IL-5 ( B ) and IL-13 ( C ) was quantified by ELISA. n = 5−7 per group.

Article Snippet: Plates were then washed and overlaid with biotinylated anti-IL-4 (clone BVD6- 24G2, Endogen), washed, and treated with a 1∶2000 dilution of avidin conjugated alkaline phosphatase (Vector Laboratories, Burlingame, CA) for 2 hrs at room temperature.

Techniques: Cell Culture, Enzyme-linked Immunosorbent Assay

Journal: PLoS ONE

Article Title: Alternaria-Induced Release of IL-18 from Damaged Airway Epithelial Cells: An NF-κB Dependent Mechanism of Th2 Differentiation?

doi: 10.1371/journal.pone.0030280

Figure Lengend Snippet: ( A,B,C ). Effect of NEMO peptide on Th2 differentiation. Negatively selected naïve CD3 + CD4 + T-cells were cultured with plate bound anti-CD3 and anti-CD28 with PBS (PBS), IL-18 100 ng/ml (IL-18), IL-18 100 ng/ml and 10 µM NEMO Binding Domain Binding Peptide (IL-18+NEMO) for the initial culture period. The cells were washed and restimulated, and secretion of IL-4 ( A ), IL-5 ( B ) and IFN-γ( C ) were quantified. The data was expressed as fold increase compared to cells cultured with PBS. ( D,E,F ). GATA3 expression in T-cells. Negatively selected naïve CD3 + CD4 + T-cells were cultured with plate bound anti-CD3 and anti-CD28 in the presence of PBS ( D ), IL-4 ( E ) or IL-18 ( F ) for 7 days. GATA3 expression was measured by flow cytometric analysis.

Article Snippet: Plates were then washed and overlaid with biotinylated anti-IL-4 (clone BVD6- 24G2, Endogen), washed, and treated with a 1∶2000 dilution of avidin conjugated alkaline phosphatase (Vector Laboratories, Burlingame, CA) for 2 hrs at room temperature.

Techniques: Cell Culture, Binding Assay, Expressing